Cleantag Small Rna Library Prep Kit

However library prep is dominated by adapter dimer formation.

Cleantag small rna library prep kit.

The total yield was 0 91 gb with 97 of bases at or above q30. Cleantag small rna library prep kit primers. Cleantag small rna library prep kit contains chemically modified adapters and reagents to convert small rna having 5 phosphate and 3 hydroxyl groups to corresponding libraries for next generation sequencing. Truseq small rna library prep kit reference guide 15004197 instructions for preparing libraries using the truseq small rna library prep kit.

The kit includes cleantag chemically modified adapters that greatly reduce adapter dimer and is optimized for total rna input from 1 1000 ng. Small rna sequencing small rna seq from single cells is a powerful analytical tool. This version of the kit is intended for use with your barcode system and please note that index primers for multiplexing are not included in this kit. Cleantag small rna library prep kit.

This limits the amount of quality data retrieved from each sample. Cleantag small rna library prep kit is compatible with illumina and ion torrent technology and contains all necessary components for small rna library preparation for next generation sequencing. In collaboration with hudson alpha institute for biotechnology we show that the cleantag ligation kit for small rna library prep efficiently tags small rna and produces significantly more valuable reads when compared to a previously published protocol optimized for adapter dimer reduction. The human brain reference rna hbrr and universal human reference rna uhrr samples were prepared using the truseq small rna library prep kit.

The nebnext multiplex small rna library prep kit for illumina index primers 1 48 contains the adaptors primers enzymes and buffers required to convert small rnas into indexed libraries for next generation sequencing on the illumina platform. These libraries were sequenced on the miniseq system using the miniseq high output reagent kit at a 1 x 36 bp read length configuration. The unique workflow of the nebnext small rna library prep kits addresses the challenge of minimization of adaptor dimers while achieving production of high yield diverse multiplex libraries in a simple protocol. Small rna library prep kits with single index barcode primers.

Multiplexing is facilitated using indexed pcr primers.